Producción científica e innovación aplicada
Avances en Nanomedicina,
Diagnóstico y Desarrollo de Fármacos
Este compendio reúne más de 100 publicaciones científicas y patentes desarrolladas por investigadores asociados al grupo ND3 – Nanomedicine, Diagnostic & Drug Development.
Abarcando desde estudios sobre regeneración muscular, terapias dirigidas con nanopartículas, mecanismos moleculares en cáncer, hasta innovadoras formulaciones farmacéuticas, estas contribuciones reflejan una sólida trayectoria en investigación traslacional e innovación tecnológica.
Las patentes incluidas complementan estos hallazgos con desarrollos aplicados en liberación controlada de fármacos y biomateriales, consolidando un aporte significativo al avance científico y clínico en salud humana y animal.
Archivo
Publicaciones 2017-2025
- Abrigo, , Simon, F., Cabrera, D., Vilos, C. & Cabello-Verrugio, C. Combined Administration of Andrographolide and Angiotensin- (1-7) Synergically Increases the Muscle Function and Strength in Aged Mice. Curr Mol Med (2021).
- Tacchi, et al. Scaffold biomaterials and nano-based therapeutic strategies for skeletal muscle regeneration. Nanomedicine (Lond) 16, 2521-2538 (2021).
- Gonzalez, et al. The Critical Role of Oxidative Stress in Sarcopenic Obesity. Oxid Med Cell Longev 2021, 4493817 (2021).
- Cabana-Brunod, et al. Development of a PHBV nanoparticle as a peptide vehicle for NOD1 activation. Drug Deliv 28, 1020-1030 (2021).
- Cisterna, A. et al. Active acetylcholine receptors prevent the atrophy of skeletal muscles and favor reinnervation. Nat Commun 11, 1073 (2020).
- Garcia-Lopez, P., Vilos, C. & Feijoo, C.G. Zebrafish, a model to develop nanotherapeutics that control neutrophils response during inflammation. J Control Release 313, 14-23 (2019).
- Abrigo, et al. N-Acetyl Cysteine Attenuates the Sarcopenia and Muscle Apoptosis Induced by Chronic Liver Disease. Curr Mol Med 20, 60-71 (2019).
- Abrigo, , Simon, F., Cabrera, D., Vilos, C. & Cabello-Verrugio, C. Mitochondrial Dysfunction in Skeletal Muscle Pathologies. Curr Protein Pept Sci 20, 536-546 (2019).
- Cabello-Verrugio, , Vilos, C., Rodrigues-Diez, R. & Estrada, L. Oxidative Stress in Disease and Aging: Mechanisms and Therapies 2018. Oxid Med Cell Longev 2018, 2835189 (2018).
- Matus, F., Luduena, M., Vilos, C., Palomo, I. & Mariscal, M.M. Atomic-level characterization and cilostazol affinity of poly(lactic acid) nanoparticles conjugated with differentially charged hydrophilic molecules. Beilstein J Nanotechnol 9, 1328-1338 (2018).
- Sahu, et al. A novel alendronate functionalized nanoprobe for simple colorimetric detection of cancer-associated hypercalcemia. Nanoscale 10, 13375-13383 (2018).
- Abrigo, et al. Role of Oxidative Stress as Key Regulator of Muscle Wasting during Cachexia.
Oxid Med Cell Longev 2018, 2063179 (2018).
- Matus, F., Vilos, C., Cisterna, B.A., Fuentes, E. & Palomo, I. Nanotechnology and primary hemostasis: Differential effects of nanoparticles on platelet responses. Vascul Pharmacol 101, 1-8 (2018).
- Abrigo, et al. TGF-beta requires the activation of canonical and non-canonical signalling pathways to induce skeletal muscle atrophy. Biol Chem 399, 253-264 (2018).
- Choi, I. et al. Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors. Sci Rep 7, 14351 (2017).
- Penaloza, P. et al. Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines. J Nanobiotechnology 15, 1 (2017).
- Pinto, P., Arce, M., Yameen, B. & Vilos, C. Targeted brain delivery nanoparticles for malignant gliomas. Nanomedicine (Lond) 12, 59-72 (2017).
- Fuentes, et al. Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles. Nanomedicine 13, 1089-1094 (2017).
- Munoz-Quezada, T. et al. Psychometric Validation of a Questionnaire to Assess Perception and Knowledge About Exposure to Pesticides in Rural Schoolchildren of Maule, Chile. Front Psychol 12, 715477 (2021).
- Zuniga, et al. Withaferin A suppresses breast cancer cell proliferation by inhibition of the two-pore domain potassium (K2P9) channel TASK-3. Biomed Pharmacother 129, 110383 (2020).
- Bustos, et al. Elucidating the Structural Basis of the Intracellular pH Sensing Mechanism of TASK-2 K2P Channels. Int J Mol Sci 21 (2020).
- Cikutovic-Molina, , Herrada, A.A., Gonzalez, W., Brown, N. & Zuniga, L. TASK-3 Gene Knockdown Dampens Invasion and Migration and Promotes Apoptosis in KATO III and MKN-45 Human Gastric Adenocarcinoma Cell Lines. Int J Mol Sci 20 (2019).
- Concha, , Bustos, D., Zuniga, R., Catalan, M.A. & Zuniga, L. Correction: Concha, G., et al. The Insensitivity of TASK-3 K2P Channels to External Tetraethylammonium (TEA) Partially Depends on the Cap Structure. Int. J. Mol. Sci. 2018, 19, 2437. Int J Mol Sci 20 (2019).
- Ramirez, et al. Discovery of Novel TASK-3 Channel Blockers Using a Pharmacophore-Based Virtual Screening. Int J Mol Sci 20 (2019).
- Munoz-Quezada, T. et al. An educational intervention on the risk perception of pesticides exposure and organophosphate metabolites urinary concentrations in rural school children in Maule Region, Chile. Environ Res 176, 108554 (2019).
- Ramirez, , Zuniga, R., Concha, G. & Zuniga, L. HCN Channels: New Therapeutic Targets for Pain Treatment. Molecules 23 (2018).
- Concha, , Bustos, D., Zuniga, R., Catalan, M.A. & Zuniga, L. The Insensitivity of TASK-3 K(2)P Channels to External Tetraethylammonium (TEA) Partially Depends on the Cap Structure. Int J Mol Sci 19 (2018).
- Mendez-Rojas, et al. Synthesis and biological evaluation of potential acetylcholinesterase inhibitors based on a benzoxazine core. Arch Pharm (Weinheim) 351, e1800024 (2018).
- Zuniga, , Valenzuela, C., Concha, G., Brown, N. & Zuniga, L. TASK-3 Downregulation Triggers Cellular Senescence and Growth Inhibition in Breast Cancer Cell Lines. Int J Mol Sci 19 (2018).
- Munoz, et al. Nanoengineered Sprayable Therapy for Treating Myocardial Infarction. ACS Nano (2022).
- Schnettler, et al. Family-to-work enrichment associations between family meal atmosphere and job satisfaction in dual-earner parents. Curr Psychol, 1-19 (2022).
- Schnettler, et al. Relationships Between Work-to-Family Conflict and the Food Domain for Dual-Earner Parents With Adolescent Children. Front Psychol 12, 752209 (2021).
- Schnettler, et al. Diet quality during the COVID-19 pandemic: Effects of workplace support for families and work-to-family enrichment in dual-earner parents with adolescent children. Appetite 169, 105823 (2022).
- McTiernan, D. et al. Molecular rotors as reporters for viscosity of solutions of collagen like peptides. Phys Chem Chem Phys 23, 24545-24549 (2021).
- Schnettler, et al. Domain Satisfaction and Overall Life Satisfaction: Testing the Spillover- Crossover Model in Chilean Dual-Earner Couples. Int J Environ Res Public Health 17 (2020).
- Rivas, et al. KCTD5, a novel TRPM4-regulatory protein required for cell migration as a new predictor for breast cancer prognosis. FASEB J 34, 7847-7865 (2020).
- McTiernan, D. et al. Light-Activated Peptide-Based Materials for Sutureless Wound Closure.
ACS Appl Mater Interfaces 11, 45007-45015 (2019).
- Vergara-Jaque, et al. A Structural Model of the Inactivation Gate of Voltage-Activated Potassium Channels. Biophys J 117, 377-387 (2019).
- Moldenhauer, , Diaz-Franulic, I., Poblete, H. & Naranjo, D. Trans-toxin ion-sensitivity of charybdotoxin-blocked potassium-channels reveals unbinding transitional states. Elife 8 (2019).
- Goel, et al. Nanoparticle Concentration vs Surface Area in the Interaction of Thiol- Containing Molecules: Toward a Rational Nanoarchitectural Design of Hybrid Materials. ACS Appl Mater Interfaces 11, 17697-17705 (2019).
- Azhagiya Singam, R. et al. Thermodynamics of Adsorption on Graphenic Surfaces from Aqueous Solution. J Chem Theory Comput 15, 1302-1316 (2019).
- Camarada, B. et al. Experimental and Computational Characterization of the Interaction between Gold Nanoparticles and Polyamidoamine Dendrimers. Langmuir 34, 10063-10072 (2018).
- Mena-Ulecia, et al. Study of the affinity between the protein kinase PKA and homoarginine-containing peptides derived from kemptide: Free energy perturbation (FEP) calculations. J Comput Chem 39, 986-992 (2018).
- Alarcon, I. et al. Rose Bengal Binding to Collagen and Tissue Photobonding. ACS Omega 2, 6646-6657 (2017).
- Carreno, et al. Development of “on-demand” thermo-responsive hydrogels for anti-cancer drugs sustained release: Rational design, in silico prediction and in vitro validation in colon cancer models. Mater Sci Eng C Mater Biol Appl 131, 112483 (2021).
- Valdes, et al. Use of Poly(vinyl alcohol)-Malic Acid (CLHPMA) Hydrogels and Chitosan Coated Calcium Alginate (CCCA) Microparticles as Potential Sorbent Phases for the Extraction and Quantitative Determination of Pesticides from Aqueous Solutions. Polymers (Basel) 13 (2021).
- Rafael, et al. Thermo-responsive hydrogels for cancer local therapy: Challenges and state- of-art. Int J Pharm 606, 120954 (2021).
- Andrade, , Roca-Melendres, M.M., Duran-Lara, E.F., Rafael, D. & Schwartz, S., Jr. Stimuli- Responsive Hydrogels for Cancer Treatment: The Role of pH, Light, Ionic Strength and Magnetic Field. Cancers (Basel) 13 (2021).
- Arauna, , Vijayakumar, S. & Duran-Lara, E. Latest Advances in Hydrogel-Based Drug Delivery Systems for Optimization of Metabolic Syndrome Treatment. Curr Med Chem 28, 6274-6286 (2021).
- Carreno, et al. Sustained Release of Linezolid from Prepared Hydrogels with Polyvinyl Alcohol and Aliphatic Dicarboxylic Acids of Variable Chain Lengths. Pharmaceutics 12 (2020).
- Marican, , Forero-Doria, O., Polo, E., Gallego, J. & Duran-Lara, E.F. Data of preparation and evaluation of supramolecular hydrogel based on cellulose for sustained release of therapeutic substances with antimicrobial and wound healing properties. Data Brief 31, 105902 (2020).
- Forero-Doria, et al. Supramolecular hydrogels based on cellulose for sustained release of therapeutic substances with antimicrobial and wound healing properties. Carbohydr Polym 242, 116383 (2020).
- Divya, , Vijayakumar, S., Chen, J., Vaseeharan, B. & Duran-Lara, E.F. South Indian medicinal plants can combat deadly viruses along with COVID-19? – A review. Microb Pathog 148, 104277 (2020).
- Vijayakumar, et al. Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles for anti MRSA therapy. Int J Biol Macromol 144, 9-18 (2020).
- Abril, et al. Comparison of the Oxidative Stability and Antioxidant Activity of Extra-Virgin Olive Oil and Oils Extracted from Seeds of Colliguaya integerrima and Cynara cardunculus Under Normal Conditions and After Thermal Treatment. Antioxidants (Basel) 8 (2019).
- Avila-Salas, et al. Film Dressings Based on Hydrogels: Simultaneous and Sustained-Release of Bioactive Compounds with Wound Healing Properties. Pharmaceutics 11 (2019).
- Vijayakumar, et al. Garlic clove extract assisted silver nanoparticle – Antibacterial, antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity assessment. J Photochem Photobiol B 198, 111558 (2019).
- Avila-Salas, & Duran-Lara, E.F. An Overview of Injectable Thermo-Responsive Hydrogels and Advances in their Biomedical Applications. Curr Med Chem 27, 5773-5789 (2020).
- Rodriguez Nunez, Y.A. et al. Preparation of Hydrogel/Silver Nanohybrids Mediated by Tunable-Size Silver Nanoparticles for Potential Antibacterial Polymers (Basel) 11 (2019).
- Avila-Salas, et al. Rational Development of a Novel Hydrogel as a pH-Sensitive Controlled Release System for Nifedipine. Polymers (Basel) 10 (2018).
- Marican, et al. Rational Design, Synthesis and Evaluation of gamma-CD-Containing Cross- Linked Polyvinyl Alcohol Hydrogel as a Prednisone Delivery Platform. Pharmaceutics 10 (2018).
- Avila-Salas, et al. In-Silico Design, Synthesis and Evaluation of a Nanostructured Hydrogel as a Dimethoate Removal Agent. Nanomaterials (Basel) 8 (2018).
- Marican, & Duran-Lara, E.F. A review on pesticide removal through different processes.
Environ Sci Pollut Res Int 25, 2051-2064 (2018).
- Vishnubhakthula, , Elupula, R. & Duran-Lara, E.F. Recent Advances in Hydrogel-Based Drug Delivery for Melanoma Cancer Therapy: A Mini Review. J Drug Deliv 2017, 7275985 (2017).
- Hernandez-Olivos, et al. Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria. Sci Rep 11, 15646 (2021).
- Chan, L. et al. Polycomb complexes associate with enhancers and promote oncogenic transcriptional programs in cancer through multiple mechanisms. Nat Commun 9, 3377 (2018).
- Usman, B. et al. Biological databases and tools for neurological disorders. J Integr Neurosci
21, 41 (2022).
- Charbe, B. et al. A new era in oxygen therapeutics? From perfluorocarbon systems to haemoglobin-based oxygen carriers. Blood Rev, 100927 (2022).
- Rodriguez, F. et al. Green by Design: Convergent Synthesis, Computational Analyses, and Activity Evaluation of New FXa Inhibitors Bearing Peptide Triazole Linking Units. Pharmaceutics 14 (2021).
- Khursheed, et al. Self-nanoemulsifying composition containing curcumin, quercetin, Ganoderma lucidum extract powder and probiotics for effective treatment of type 2 diabetes mellitus in streptozotocin induced rats. Int J Pharm 612, 121306 (2022).
- Prasher, et al. Recent Trends in rationally designed molecules as kinase inhibitors. Curr Med Chem (2021).
- Chellappan, K. et al. Protein and peptide delivery to lungs by using advanced targeted drug delivery. Chem Biol Interact 351, 109706 (2022).
- Khursheed, et al. Development of mushroom polysaccharide and probiotics based solid self-nanoemulsifying drug delivery system loaded with curcumin and quercetin to improve their dissolution rate and permeability: State of the art. Int J Biol Macromol 189, 744-757 (2021).
- Satija, et al. Perfluorocarbons Therapeutics in Modern Cancer Nanotechnology for Hypoxiainduced Anti-tumor Therapy. Curr Pharm Des 27, 4376-4387 (2021).
- Viteri, , Giordano, A., Montenegro, G. & Zacconi, F. Eucryphia cordifolia extracts: Phytochemical screening, antibacterial and antioxidant activities. Nat Prod Res, 1-5 (2021).
- Chan, et al. Advances in nanotechnology-based drug delivery in targeting PI3K signaling in respiratory diseases. Nanomedicine (Lond) 16, 1351-1355 (2021).
- Xavier, et al. Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy. Ann Hepatol 25, 100358 (2021).
- Viteri, , Zacconi, F., Montenegro, G. & Giordano, A. Bioactive compounds in Apis mellifera monofloral honeys. J Food Sci 86, 1552-1582 (2021).
- Mehta, et al. Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation. Future Med Chem 13, 543-549 (2021).
- Satija, et al. Hypoxia-Inducible Factor (HIF): Fuel for Cancer Progression. Curr Mol Pharmacol 14, 321-332 (2021).
- Lee, Y. et al. Targeting eosinophils in respiratory diseases: Biological axis, emerging therapeutics and treatment modalities. Life Sci 267, 118973 (2021).
- Charbe, B. et al. Small interfering RNA for cancer treatment: overcoming hurdles in delivery. Acta Pharm Sin B 10, 2075-2109 (2020).
- Borrero-de Acuna, J.M. et al. Channelling carbon flux through the meta-cleavage route for improved poly(3-hydroxyalkanoate) production from benzoate and lignin-based aromatics in Pseudomonas putida H. Microb Biotechnol 14, 2385-2402 (2021).
- Sharma, et al. Probing 3CL protease: Rationally designed chemical moieties for COVID-19.
Drug Dev Res (2020).
- Prasher, et al. Hybrid molecules based on 1,3,5-triazine as potential therapeutics: A focused review. Drug Dev Res 81, 837-858 (2020).
- Santana-Romo, et al. Innovative Three-Step Microwave-Promoted Synthesis of N- Propargyltetrahydroquinoline and 1,2,3-Triazole Derivatives as a Potential Factor Xa (FXa) Inhibitors: Drug Design, Synthesis, and Biological Evaluation. Molecules 25 (2020).
- Herrera, et al. [Quantification of liver fat infiltration by magnetic resonance]. Rev Med Chil
147, 821-827 (2019).
- Zaupa, et al. Cold-adaptation of a methacrylamide gelatin towards the expansion of the biomaterial toolbox for specialized functionalities in tissue engineering. Mater Sci Eng C Mater Biol Appl 102, 373-390 (2019).
- Pacheco, et al. Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers. J Ind Microbiol Biotechnol 46, 1139-1153 (2019).
- Duran, , Nunez-Navarro, N., Zacconi, F.C. & Herrera, B. Theoretical study of C-arylations with aryl halides to determine the reaction mechanism, the effect of substituents and heteroatoms. Phys Chem Chem Phys 21, 10163-10170 (2019).
- Charbe, B. et al. Emergence of Three Dimensional Printed Cardiac Tissue: Opportunities and Challenges in Cardiovascular Diseases. Curr Cardiol Rev 15, 188-204 (2019).
- Nunez-Navarro, E., Santana, F.M., Parra, L.P. & Zacconi, F.C. Surfing the Blood Coagulation Cascade: Insight into the Vital Factor Xa. Curr Med Chem 26, 3175-3200 (2019).
- Oliva-Arancibia, et al. Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440. J Biotechnol 264, 29-37 (2017).
- Lagos, F., Segovia, G.F., Nunez-Navarro, N., Faundez, M.A. & Zacconi, F.C. Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches. Molecules 22 (2017).
- Padilla, et al. Chitosan/poly-octanoic acid 2-thiophen-3-yl-ethyl ester blends as a scaffold to maintain myoblasts regeneration potential in vitro. J Biomed Mater Res A 105, 118-130 (2017).
- Bachler, et al. [Effectiveness of a structured educative program in Chilean diabetic patients]. Rev Med Chil 145, 181-187 (2017).
- Vergara-Jaque, , Fong, P. & Comer, J. Iodide Binding in Sodium-Coupled Cotransporters. J Chem Inf Model 57, 3043-3055 (2017).
- Carrasco-Sanchez, et al. Polymeric substances for the removal of ochratoxin A from red wine followed by computational modeling of the complexes formed. Food Chem 265, 159-164 (2018).
- Blanco, et al. EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion. FASEB J 33, 9434-9452 (2019).
- Dreyer, , Vergara-Jaque, A., Riedelsberger, J. & Gonzalez, W. Exploring the fundamental role of potassium channels in novel model plants. J Exp Bot 70, 5985-5989 (2019).
- Riedelsberger, , Vergara-Jaque, A., Pineros, M., Dreyer, I. & Gonzalez, W. An extracellular cation coordination site influences ion conduction of OsHKT2;2. BMC Plant Biol 19, 316 (2019).
- Rojas, et al. Structural Determinants of the Dopamine Transporter Regulation Mediated by G Proteins. J Chem Inf Model 60, 3577-3586 (2020).
- Rossino, G. et al. New Insights into the Opening of the Occluded Ligand-Binding Pocket of Sigma1 Receptor: Binding of a Novel Bivalent RC-33 J Chem Inf Model 60, 756-765 (2020).
- Sarkar, K. et al. A transient amphipathic helix in the prodomain of PCSK9 facilitates binding to low-density lipoprotein particles. J Biol Chem 295, 2285-2298 (2020).
- Comer, et al. Beta-1,3 Oligoglucans Specifically Bind to Immune Receptor CD28 and May Enhance T Cell Activation. Int J Mol Sci 22 (2021).
- Fernandez, C. et TMPRSS11a is a novel age-altered, tissue specific regulator of migration and wound healing. FASEB J 35, e21597 (2021).
- Gonzalez-Avendano, et al. PPI-MASS: An Interactive Web Server to Identify Protein-Protein Interactions From Mass Spectrometry-Based Proteomics Data. Front Mol Biosci 8, 701477 (2021).
- Rossino, G. et al. Bitopic Sigma 1 Receptor Modulators to Shed Light on Molecular Mechanisms Underpinning Ligand Binding and Receptor J Med Chem 64, 14997-15016 (2021).
- Pena-Varas, et al. Structural Insights into the Substrate Transport Mechanisms in GTR Transporters through Ensemble Docking. Int J Mol Sci 23 (2022).
Patentes 2017-2025
- Gerner E, Paz E, Lafleur B, Garcia-Huidobro J. Predictive Markers for Polyamine Inhibitor Cancer Therapies PCT/US10151756B2. Published and Granted 2020-11-24 (12 paises y en proceso).
- Gerner E, Brooks T, Thompson P, Garcia-Huidobro J. Carcinoma diagnosis and treatment, based on ODC1 genotype PCT/US20170362658A1. Published and Granted 2020-05-19 (4 paises y en proceso).
- SHIEKHATTAR R, BLUMENTHAL E, GARCIA-HUIDOBRO J. INTEGRATOR INHIBITORS AND METHODS FOR THEIR USE PCT/US20190358220A1. PUBLISHED 2019-11-28 AND PENDING.
- Constandil L., Ibarra P., Vilos C., Veláquez L., Pelissier T., Laurido C., Hernández A. Nanopartículas con polímero biodegradable y biocompatible PLGA cargadas con el fármaco de uso humano pentoxifilina. Número de Solicitud 3209-2012/16.11.2012.
- Shi J., Zeyu X., Votruba A., Vilos C., Farokhzad O. United States Patent, entitled “LIPID- POLYMER HYBRID PARTICLES,” filed on September 3, 2010, and assigned U.S. Serial Number 61/380,095. (US Provisional ).
- Vilos C., Velásquez L. A., Orihuela P., Ramirez M. Composición Inyectable de liberación prolongada de Ceftiofur y Florfenicol en especies animales que comprende micropartículas de ácido PoliLáctico-Glicólico (PLGA) y procedimiento de preparación (Injectable sustained release composition of ceftiofur and florfenicol in animal species comprising microparticles of polylactic- glycolic acid (PLGA) and method of preparation). National Institute of Industrial Property (INAPI), Chile Provisional 2902-2011.
- Vilos C., Velásquez L. A., Constandil L., Ramirez R. Composición Inyectable de liberación prolongada de Ceftiofur y Florfenicol en especies animales que comprende micropartículas de ácido Polihidroxibutirato-hidroxivalerato (PHBV) y procedimiento de preparación (Injectable sustained release composition of ceftiofur and florfenicol in animal species comprising microparticles of polyhydroxybutyrate-hydroxyvalerate acid (PHBV) and method of preparation). National Institute of Industrial Property (INAPI), Chile Provisional 2901-2011.