Producción científica e innovación aplicada

Publicaciones y Patentes

Avances en Nanomedicina,
Diagnóstico y Desarrollo de Fármacos

Este compendio reúne más de 100 publicaciones científicas y patentes desarrolladas por investigadores asociados al grupo ND3 – Nanomedicine, Diagnostic & Drug Development.

Abarcando desde estudios sobre regeneración muscular, terapias dirigidas con nanopartículas, mecanismos moleculares en cáncer, hasta innovadoras formulaciones farmacéuticas, estas contribuciones reflejan una sólida trayectoria en investigación traslacional e innovación tecnológica.

Las patentes incluidas complementan estos hallazgos con desarrollos aplicados en liberación controlada de fármacos y biomateriales, consolidando un aporte significativo al avance científico y clínico en salud humana y animal.

Archivo

  1. Abrigo, , Simon, F., Cabrera, D., Vilos, C. & Cabello-Verrugio, C. Combined Administration of Andrographolide and Angiotensin- (1-7) Synergically Increases the Muscle Function and Strength in Aged Mice. Curr Mol Med (2021).
  2. Tacchi, et al. Scaffold biomaterials and nano-based therapeutic strategies for skeletal muscle regeneration. Nanomedicine (Lond) 16, 2521-2538 (2021).
  3. Gonzalez, et al. The Critical Role of Oxidative Stress in Sarcopenic Obesity. Oxid Med Cell Longev 2021, 4493817 (2021).
  4. Cabana-Brunod, et al. Development of a PHBV nanoparticle as a peptide vehicle for NOD1 activation. Drug Deliv 28, 1020-1030 (2021).
  5. Cisterna, A. et al. Active acetylcholine receptors prevent the atrophy of skeletal muscles and favor reinnervation. Nat Commun 11, 1073 (2020).
  6. Garcia-Lopez, P., Vilos, C. & Feijoo, C.G. Zebrafish, a model to develop nanotherapeutics that control neutrophils response during inflammation. J Control Release 313, 14-23 (2019).
  7. Abrigo, et al. N-Acetyl Cysteine Attenuates the Sarcopenia and Muscle Apoptosis Induced by Chronic Liver Disease. Curr Mol Med 20, 60-71 (2019).
  8. Abrigo, , Simon, F., Cabrera, D., Vilos, C. & Cabello-Verrugio, C. Mitochondrial Dysfunction in Skeletal Muscle Pathologies. Curr Protein Pept Sci 20, 536-546 (2019).
  9. Cabello-Verrugio, , Vilos, C., Rodrigues-Diez, R. & Estrada, L. Oxidative Stress in Disease and Aging: Mechanisms and Therapies 2018. Oxid Med Cell Longev 2018, 2835189 (2018).
  10. Matus, F., Luduena, M., Vilos, C., Palomo, I. & Mariscal, M.M. Atomic-level characterization and cilostazol affinity of poly(lactic acid) nanoparticles conjugated with differentially charged hydrophilic molecules. Beilstein J Nanotechnol 9, 1328-1338 (2018).
  11. Sahu, et al. A novel alendronate functionalized nanoprobe for simple colorimetric detection of cancer-associated hypercalcemia. Nanoscale 10, 13375-13383 (2018).
  12. Abrigo, et al. Role of Oxidative Stress as Key Regulator of Muscle Wasting during Cachexia.

Oxid Med Cell Longev 2018, 2063179 (2018).

  1. Matus, F., Vilos, C., Cisterna, B.A., Fuentes, E. & Palomo, I. Nanotechnology and primary hemostasis: Differential effects of nanoparticles on platelet responses. Vascul Pharmacol 101, 1-8 (2018).
  2. Abrigo, et al. TGF-beta requires the activation of canonical and non-canonical signalling pathways to induce skeletal muscle atrophy. Biol Chem 399, 253-264 (2018).
  3. Choi, I. et al. Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors. Sci Rep 7, 14351 (2017).
  4. Penaloza, P. et al. Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines. J Nanobiotechnology 15, 1 (2017).
  5. Pinto, P., Arce, M., Yameen, B. & Vilos, C. Targeted brain delivery nanoparticles for malignant gliomas. Nanomedicine (Lond) 12, 59-72 (2017).
  6. Fuentes, et al. Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles. Nanomedicine 13, 1089-1094 (2017).
  1. Munoz-Quezada, T. et al. Psychometric Validation of a Questionnaire to Assess Perception and Knowledge About Exposure to Pesticides in Rural Schoolchildren of Maule, Chile. Front Psychol 12, 715477 (2021).
  2. Zuniga, et al. Withaferin A suppresses breast cancer cell proliferation by inhibition of the two-pore domain potassium (K2P9) channel TASK-3. Biomed Pharmacother 129, 110383 (2020).
  3. Bustos, et al. Elucidating the Structural Basis of the Intracellular pH Sensing Mechanism of TASK-2 K2P Channels. Int J Mol Sci 21 (2020).
  4. Cikutovic-Molina, , Herrada, A.A., Gonzalez, W., Brown, N. & Zuniga, L. TASK-3 Gene Knockdown Dampens Invasion and Migration and Promotes Apoptosis in KATO III and MKN-45 Human Gastric Adenocarcinoma Cell Lines. Int J Mol Sci 20 (2019).
  5. Concha, , Bustos, D., Zuniga, R., Catalan, M.A. & Zuniga, L. Correction: Concha, G., et al. The Insensitivity of TASK-3 K2P Channels to External Tetraethylammonium (TEA) Partially Depends on the Cap Structure. Int. J. Mol. Sci. 2018, 19, 2437. Int J Mol Sci 20 (2019).
  6. Ramirez, et al. Discovery of Novel TASK-3 Channel Blockers Using a Pharmacophore-Based Virtual Screening. Int J Mol Sci 20 (2019).
  7. Munoz-Quezada, T. et al. An educational intervention on the risk perception of pesticides exposure and organophosphate metabolites urinary concentrations in rural school children in Maule Region, Chile. Environ Res 176, 108554 (2019).
  8. Ramirez, , Zuniga, R., Concha, G. & Zuniga, L. HCN Channels: New Therapeutic Targets for Pain Treatment. Molecules 23 (2018).
  9. Concha, , Bustos, D., Zuniga, R., Catalan, M.A. & Zuniga, L. The Insensitivity of TASK-3 K(2)P Channels to External Tetraethylammonium (TEA) Partially Depends on the Cap Structure. Int J Mol Sci 19 (2018).
  10. Mendez-Rojas, et al. Synthesis and biological evaluation of potential acetylcholinesterase inhibitors based on a benzoxazine core. Arch Pharm (Weinheim) 351, e1800024 (2018).
  11. Zuniga, , Valenzuela, C., Concha, G., Brown, N. & Zuniga, L. TASK-3 Downregulation Triggers Cellular Senescence and Growth Inhibition in Breast Cancer Cell Lines. Int J Mol Sci 19 (2018).
  12. Munoz, et al. Nanoengineered Sprayable Therapy for Treating Myocardial Infarction. ACS Nano (2022).
  13. Schnettler, et al. Family-to-work enrichment associations between family meal atmosphere and job satisfaction in dual-earner parents. Curr Psychol, 1-19 (2022).
  14. Schnettler, et al. Relationships Between Work-to-Family Conflict and the Food Domain for Dual-Earner Parents With Adolescent Children. Front Psychol 12, 752209 (2021).
  15. Schnettler, et al. Diet quality during the COVID-19 pandemic: Effects of workplace support for families and work-to-family enrichment in dual-earner parents with adolescent children. Appetite 169, 105823 (2022).
  16. McTiernan, D. et al. Molecular rotors as reporters for viscosity of solutions of collagen like peptides. Phys Chem Chem Phys 23, 24545-24549 (2021).
  17. Schnettler, et al. Domain Satisfaction and Overall Life Satisfaction: Testing the Spillover- Crossover Model in Chilean Dual-Earner Couples. Int J Environ Res Public Health 17 (2020).
  18. Rivas, et al. KCTD5, a novel TRPM4-regulatory protein required for cell migration as a new predictor for breast cancer prognosis. FASEB J 34, 7847-7865 (2020).
  19. McTiernan, D. et al. Light-Activated Peptide-Based Materials for Sutureless Wound Closure.

ACS Appl Mater Interfaces 11, 45007-45015 (2019).

  1. Vergara-Jaque, et al. A Structural Model of the Inactivation Gate of Voltage-Activated Potassium Channels. Biophys J 117, 377-387 (2019).
  2. Moldenhauer, , Diaz-Franulic, I., Poblete, H. & Naranjo, D. Trans-toxin ion-sensitivity of charybdotoxin-blocked potassium-channels reveals unbinding transitional states. Elife 8 (2019).
  3. Goel, et al. Nanoparticle Concentration vs Surface Area in the Interaction of Thiol- Containing Molecules: Toward a Rational Nanoarchitectural Design of Hybrid Materials. ACS Appl Mater Interfaces 11, 17697-17705 (2019).
  4. Azhagiya Singam, R. et al. Thermodynamics of Adsorption on Graphenic Surfaces from Aqueous Solution. J Chem Theory Comput 15, 1302-1316 (2019).
  5. Camarada, B. et al. Experimental and Computational Characterization of the Interaction between Gold Nanoparticles and Polyamidoamine Dendrimers. Langmuir 34, 10063-10072 (2018).
  6. Mena-Ulecia, et al. Study of the affinity between the protein kinase PKA and homoarginine-containing peptides derived from kemptide: Free energy perturbation (FEP) calculations. J Comput Chem 39, 986-992 (2018).
  7. Alarcon, I. et al. Rose Bengal Binding to Collagen and Tissue Photobonding. ACS Omega 2, 6646-6657 (2017).
  8. Carreno, et al. Development of “on-demand” thermo-responsive hydrogels for anti-cancer drugs sustained release: Rational design, in silico prediction and in vitro validation in colon cancer models. Mater Sci Eng C Mater Biol Appl 131, 112483 (2021).
  9. Valdes, et al. Use of Poly(vinyl alcohol)-Malic Acid (CLHPMA) Hydrogels and Chitosan Coated Calcium Alginate (CCCA) Microparticles as Potential Sorbent Phases for the Extraction and Quantitative Determination of Pesticides from Aqueous Solutions. Polymers (Basel) 13 (2021).
  10. Rafael, et al. Thermo-responsive hydrogels for cancer local therapy: Challenges and state- of-art. Int J Pharm 606, 120954 (2021).
  11. Andrade, , Roca-Melendres, M.M., Duran-Lara, E.F., Rafael, D. & Schwartz, S., Jr. Stimuli- Responsive Hydrogels for Cancer Treatment: The Role of pH, Light, Ionic Strength and Magnetic Field. Cancers (Basel) 13 (2021).
  12. Arauna, , Vijayakumar, S. & Duran-Lara, E. Latest Advances in Hydrogel-Based Drug Delivery Systems for Optimization of Metabolic Syndrome Treatment. Curr Med Chem 28, 6274-6286 (2021).
  13. Carreno, et al. Sustained Release of Linezolid from Prepared Hydrogels with Polyvinyl Alcohol and Aliphatic Dicarboxylic Acids of Variable Chain Lengths. Pharmaceutics 12 (2020).
  14. Marican, , Forero-Doria, O., Polo, E., Gallego, J. & Duran-Lara, E.F. Data of preparation and evaluation of supramolecular hydrogel based on cellulose for sustained release of therapeutic substances with antimicrobial and wound healing properties. Data Brief 31, 105902 (2020).
  15. Forero-Doria, et al. Supramolecular hydrogels based on cellulose for sustained release of therapeutic substances with antimicrobial and wound healing properties. Carbohydr Polym 242, 116383 (2020).
  16. Divya, , Vijayakumar, S., Chen, J., Vaseeharan, B. & Duran-Lara, E.F. South Indian medicinal plants can combat deadly viruses along with COVID-19? – A review. Microb Pathog 148, 104277 (2020).
  1. Vijayakumar, et al. Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles for anti MRSA therapy. Int J Biol Macromol 144, 9-18 (2020).
  2. Abril, et al. Comparison of the Oxidative Stability and Antioxidant Activity of Extra-Virgin Olive Oil and Oils Extracted from Seeds of Colliguaya integerrima and Cynara cardunculus Under Normal Conditions and After Thermal Treatment. Antioxidants (Basel) 8 (2019).
  3. Avila-Salas, et al. Film Dressings Based on Hydrogels: Simultaneous and Sustained-Release of Bioactive Compounds with Wound Healing Properties. Pharmaceutics 11 (2019).
  4. Vijayakumar, et al. Garlic clove extract assisted silver nanoparticle – Antibacterial, antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity assessment. J Photochem Photobiol B 198, 111558 (2019).
  5. Avila-Salas, & Duran-Lara, E.F. An Overview of Injectable Thermo-Responsive Hydrogels and Advances in their Biomedical Applications. Curr Med Chem 27, 5773-5789 (2020).
  6. Rodriguez Nunez, Y.A. et al. Preparation of Hydrogel/Silver Nanohybrids Mediated by Tunable-Size Silver Nanoparticles for Potential Antibacterial Polymers (Basel) 11 (2019).
  7. Avila-Salas, et al. Rational Development of a Novel Hydrogel as a pH-Sensitive Controlled Release System for Nifedipine. Polymers (Basel) 10 (2018).
  8. Marican, et al. Rational Design, Synthesis and Evaluation of gamma-CD-Containing Cross- Linked Polyvinyl Alcohol Hydrogel as a Prednisone Delivery Platform. Pharmaceutics 10 (2018).
  9. Avila-Salas, et al. In-Silico Design, Synthesis and Evaluation of a Nanostructured Hydrogel as a Dimethoate Removal Agent. Nanomaterials (Basel) 8 (2018).
  10. Marican, & Duran-Lara, E.F. A review on pesticide removal through different processes.

Environ Sci Pollut Res Int 25, 2051-2064 (2018).

  1. Vishnubhakthula, , Elupula, R. & Duran-Lara, E.F. Recent Advances in Hydrogel-Based Drug Delivery for Melanoma Cancer Therapy: A Mini Review. J Drug Deliv 2017, 7275985 (2017).
  2. Hernandez-Olivos, et al. Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria. Sci Rep 11, 15646 (2021).
  3. Chan, L. et al. Polycomb complexes associate with enhancers and promote oncogenic transcriptional programs in cancer through multiple mechanisms. Nat Commun 9, 3377 (2018).
  4. Usman, B. et al. Biological databases and tools for neurological disorders. J Integr Neurosci

21, 41 (2022).

  1. Charbe, B. et al. A new era in oxygen therapeutics? From perfluorocarbon systems to haemoglobin-based oxygen carriers. Blood Rev, 100927 (2022).
  2. Rodriguez, F. et al. Green by Design: Convergent Synthesis, Computational Analyses, and Activity Evaluation of New FXa Inhibitors Bearing Peptide Triazole Linking Units. Pharmaceutics 14 (2021).
  3. Khursheed, et al. Self-nanoemulsifying composition containing curcumin, quercetin, Ganoderma lucidum extract powder and probiotics for effective treatment of type 2 diabetes mellitus in streptozotocin induced rats. Int J Pharm 612, 121306 (2022).
  4. Prasher, et al. Recent Trends in rationally designed molecules as kinase inhibitors. Curr Med Chem (2021).
  5. Chellappan, K. et al. Protein and peptide delivery to lungs by using advanced targeted drug delivery. Chem Biol Interact 351, 109706 (2022).
  1. Khursheed, et al. Development of mushroom polysaccharide and probiotics based solid self-nanoemulsifying drug delivery system loaded with curcumin and quercetin to improve their dissolution rate and permeability: State of the art. Int J Biol Macromol 189, 744-757 (2021).
  2. Satija, et al. Perfluorocarbons Therapeutics in Modern Cancer Nanotechnology for Hypoxiainduced Anti-tumor Therapy. Curr Pharm Des 27, 4376-4387 (2021).
  3. Viteri, , Giordano, A., Montenegro, G. & Zacconi, F. Eucryphia cordifolia extracts: Phytochemical screening, antibacterial and antioxidant activities. Nat Prod Res, 1-5 (2021).
  4. Chan, et al. Advances in nanotechnology-based drug delivery in targeting PI3K signaling in respiratory diseases. Nanomedicine (Lond) 16, 1351-1355 (2021).
  5. Xavier, et al. Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy. Ann Hepatol 25, 100358 (2021).
  6. Viteri, , Zacconi, F., Montenegro, G. & Giordano, A. Bioactive compounds in Apis mellifera monofloral honeys. J Food Sci 86, 1552-1582 (2021).
  7. Mehta, et al. Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation. Future Med Chem 13, 543-549 (2021).
  8. Satija, et al. Hypoxia-Inducible Factor (HIF): Fuel for Cancer Progression. Curr Mol Pharmacol 14, 321-332 (2021).
  9. Lee, Y. et al. Targeting eosinophils in respiratory diseases: Biological axis, emerging therapeutics and treatment modalities. Life Sci 267, 118973 (2021).
  10. Charbe, B. et al. Small interfering RNA for cancer treatment: overcoming hurdles in delivery. Acta Pharm Sin B 10, 2075-2109 (2020).
  11. Borrero-de Acuna, J.M. et al. Channelling carbon flux through the meta-cleavage route for improved poly(3-hydroxyalkanoate) production from benzoate and lignin-based aromatics in Pseudomonas putida H. Microb Biotechnol 14, 2385-2402 (2021).
  12. Sharma, et al. Probing 3CL protease: Rationally designed chemical moieties for COVID-19.

Drug Dev Res (2020).

  1. Prasher, et al. Hybrid molecules based on 1,3,5-triazine as potential therapeutics: A focused review. Drug Dev Res 81, 837-858 (2020).
  2. Santana-Romo, et al. Innovative Three-Step Microwave-Promoted Synthesis of N- Propargyltetrahydroquinoline and 1,2,3-Triazole Derivatives as a Potential Factor Xa (FXa) Inhibitors: Drug Design, Synthesis, and Biological Evaluation. Molecules 25 (2020).
  3. Herrera, et al. [Quantification of liver fat infiltration by magnetic resonance]. Rev Med Chil

147, 821-827 (2019).

  1. Zaupa, et al. Cold-adaptation of a methacrylamide gelatin towards the expansion of the biomaterial toolbox for specialized functionalities in tissue engineering. Mater Sci Eng C Mater Biol Appl 102, 373-390 (2019).
  2. Pacheco, et al. Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers. J Ind Microbiol Biotechnol 46, 1139-1153 (2019).
  3. Duran, , Nunez-Navarro, N., Zacconi, F.C. & Herrera, B. Theoretical study of C-arylations with aryl halides to determine the reaction mechanism, the effect of substituents and heteroatoms. Phys Chem Chem Phys 21, 10163-10170 (2019).
  4. Charbe, B. et al. Emergence of Three Dimensional Printed Cardiac Tissue: Opportunities and Challenges in Cardiovascular Diseases. Curr Cardiol Rev 15, 188-204 (2019).
  1. Nunez-Navarro, E., Santana, F.M., Parra, L.P. & Zacconi, F.C. Surfing the Blood Coagulation Cascade: Insight into the Vital Factor Xa. Curr Med Chem 26, 3175-3200 (2019).
  2. Oliva-Arancibia, et al. Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440. J Biotechnol 264, 29-37 (2017).
  3. Lagos, F., Segovia, G.F., Nunez-Navarro, N., Faundez, M.A. & Zacconi, F.C. Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches. Molecules 22 (2017).
  4. Padilla, et al. Chitosan/poly-octanoic acid 2-thiophen-3-yl-ethyl ester blends as a scaffold to maintain myoblasts regeneration potential in vitro. J Biomed Mater Res A 105, 118-130 (2017).
  5. Bachler, et al. [Effectiveness of a structured educative program in Chilean diabetic patients]. Rev Med Chil 145, 181-187 (2017).
  6. Vergara-Jaque, , Fong, P. & Comer, J. Iodide Binding in Sodium-Coupled Cotransporters. J Chem Inf Model 57, 3043-3055 (2017).
  7. Carrasco-Sanchez, et al. Polymeric substances for the removal of ochratoxin A from red wine followed by computational modeling of the complexes formed. Food Chem 265, 159-164 (2018).
  8. Blanco, et al. EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion. FASEB J 33, 9434-9452 (2019).
  9. Dreyer, , Vergara-Jaque, A., Riedelsberger, J. & Gonzalez, W. Exploring the fundamental role of potassium channels in novel model plants. J Exp Bot 70, 5985-5989 (2019).
  10. Riedelsberger, , Vergara-Jaque, A., Pineros, M., Dreyer, I. & Gonzalez, W. An extracellular cation coordination site influences ion conduction of OsHKT2;2. BMC Plant Biol 19, 316 (2019).
  11. Rojas, et al. Structural Determinants of the Dopamine Transporter Regulation Mediated by G Proteins. J Chem Inf Model 60, 3577-3586 (2020).
  12. Rossino, G. et al. New Insights into the Opening of the Occluded Ligand-Binding Pocket of Sigma1 Receptor: Binding of a Novel Bivalent RC-33 J Chem Inf Model 60, 756-765 (2020).
  13. Sarkar, K. et al. A transient amphipathic helix in the prodomain of PCSK9 facilitates binding to low-density lipoprotein particles. J Biol Chem 295, 2285-2298 (2020).
  14. Comer, et al. Beta-1,3 Oligoglucans Specifically Bind to Immune Receptor CD28 and May Enhance T Cell Activation. Int J Mol Sci 22 (2021).
  15. Fernandez, C. et TMPRSS11a is a novel age-altered, tissue specific regulator of migration and wound healing. FASEB J 35, e21597 (2021).
  16. Gonzalez-Avendano, et al. PPI-MASS: An Interactive Web Server to Identify Protein-Protein Interactions From Mass Spectrometry-Based Proteomics Data. Front Mol Biosci 8, 701477 (2021).
  17. Rossino, G. et al. Bitopic Sigma 1 Receptor Modulators to Shed Light on Molecular Mechanisms Underpinning Ligand Binding and Receptor J Med Chem 64, 14997-15016 (2021).
  18. Pena-Varas, et al. Structural Insights into the Substrate Transport Mechanisms in GTR Transporters through Ensemble Docking. Int J Mol Sci 23 (2022).
  1. Gerner E, Paz E, Lafleur B, Garcia-Huidobro J. Predictive Markers for Polyamine Inhibitor Cancer Therapies PCT/US10151756B2. Published and Granted 2020-11-24 (12 paises y en proceso).
  2. Gerner E, Brooks T, Thompson P, Garcia-Huidobro J. Carcinoma diagnosis and treatment, based on ODC1 genotype PCT/US20170362658A1. Published and Granted 2020-05-19 (4 paises y en proceso).
  3. SHIEKHATTAR R, BLUMENTHAL E, GARCIA-HUIDOBRO J. INTEGRATOR INHIBITORS AND METHODS FOR THEIR USE PCT/US20190358220A1. PUBLISHED 2019-11-28 AND PENDING.
  4. Constandil L., Ibarra P., Vilos C., Veláquez L., Pelissier T., Laurido C., Hernández A. Nanopartículas con polímero biodegradable y biocompatible PLGA cargadas con el fármaco de uso humano pentoxifilina. Número de Solicitud 3209-2012/16.11.2012.
  5. Shi J., Zeyu X., Votruba A., Vilos C., Farokhzad O. United States Patent, entitled “LIPID- POLYMER HYBRID PARTICLES,” filed on September 3, 2010, and assigned U.S. Serial Number 61/380,095. (US Provisional ).
  6. Vilos C., Velásquez L. A., Orihuela P., Ramirez M. Composición Inyectable de liberación prolongada de Ceftiofur y Florfenicol en especies animales que comprende micropartículas de ácido PoliLáctico-Glicólico (PLGA) y procedimiento de preparación (Injectable sustained release composition of ceftiofur and florfenicol in animal species comprising microparticles of polylactic- glycolic acid (PLGA) and method of preparation). National Institute of Industrial Property (INAPI), Chile Provisional 2902-2011.
  7. Vilos C., Velásquez L. A., Constandil L., Ramirez R. Composición Inyectable de liberación prolongada de Ceftiofur y Florfenicol en especies animales que comprende micropartículas de ácido Polihidroxibutirato-hidroxivalerato (PHBV) y procedimiento de preparación (Injectable sustained release composition of ceftiofur and florfenicol in animal species comprising microparticles of polyhydroxybutyrate-hydroxyvalerate acid (PHBV) and method of preparation). National Institute of Industrial Property (INAPI), Chile Provisional 2901-2011.